US7339027B2 - Human prolactin antagonist-angiogenesis inhibitor fusion proteins - Google Patents
Human prolactin antagonist-angiogenesis inhibitor fusion proteins Download PDFInfo
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- US7339027B2 US7339027B2 US10/449,609 US44960903A US7339027B2 US 7339027 B2 US7339027 B2 US 7339027B2 US 44960903 A US44960903 A US 44960903A US 7339027 B2 US7339027 B2 US 7339027B2
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A—HUMAN NECESSITIES
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
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- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
Abstract
Description
TABLE 1* | |||||
Peptide | |||||
Species | Domain | Sequence | 129 | Pep. Seq. | |
Human | PRL | IEEQTKRLLR | G | MELIVS-QVHP | |
Rat | PRL | IEEQNKRLLE | G | IEKIIG-QAYP | |
Mouse | PRL | IEEQNKQLLE | G | VEKIIS-QAYP | |
Hamster | PRL | IGEQNKRLLE | G | IEKILG-QAYP | |
Fin whale | PRL | EEEENKRLLE | G | MEKIVG-QVHP | |
Mink | PRL | IEEENRRLLE | G | MEKIVG-QVHP | |
Cattle | PRL | IEEQNKRLIE | G | MEMIFG-QVIP | |
Sheep | PRL | EEEENKRLLE | G | MENIFG-QVIP | |
Pig | PRL | IEEQNKRLLE | G | MEKIVG-QVHP | |
Camel | PRL | IEEQNKRLLE | G | MEKIVG-QVHP | |
Horse | PRL | EIEQNRRLLE | G | MEKIVG-QVQP | |
Elephant | PRL | VKEENQRLLE | G | IEKIVD-QVHP | |
Ancestral mammal | PRL | IEEENKRLLE | G | MEKIVG-QVHP | |
Chicken | PRL | IEEQNKRLLE | G | MEKIVG-RVHS | |
Turkey | PRL | IEEQDKRLLE | G | MEKIVG-RIHS | |
Sea turtle | PRL | IEEQNKRLLE | G | MEKIVG-QVHP | |
Crocodile | PRL | IEEQNKRLLE | G | MEKIIG-RVQP | |
Alligator | PRL | IEEQNKRLLE | G | MEKVIG-RVQP | |
Ancestral amniote | PRL | IEEQNKRLLE | G | MEKIVG-QVHP | |
Xenopus | PRL | VEEQNKRLLE | G | MEKIVG-RIHP | |
Bullfrog | PRL | VEEQTKRLLE | G | MERIIG-RIQP | |
Lungfish | PRL | VEDQTKQLIE | G | MEKILS-RMHP | |
Tilapia | PRL | MQQYSKSLKD | G | LD-VLSSKMGS | |
Tilapia | PRL | MQEHSKDLKD | G | LD-ILSSKMGP | |
Common carp | PRL | LQENINSLGA | G | LEHVF-NKMDS | |
Bighead carp | PRL | LQDNINSLGA | G | LERVV-HKMGS | |
Silver carp | PRL | LQDNINSLVP | G | LEHVV-HKMGS | |
Chun salmon | PRL | LQDYSKSLGD | G | LD-IMVNKMGP | |
Chinook salmon | PRL | LQDYSKSLGD | G | LD-IMVNKMGP | |
Trout | PRL | LQDYSKSLGD | G | LD-IMVNKMGP | |
Peptide | |||||
Species | Domain | Sequence | 120 | Pep. Seq. | |
Human | GH | VYDLLKDLEE | G | IQTLMRELEDG | |
Bovine | GH | VYEKLKDLEE | G | ILALMRELEDG | |
*Table 1 This table has been reproduced from Cooke et al. Journal of Biological Chemistry, 256:4007-4016 (1981). We note that this table provides that the amino acid in position number 128 of the human prolactin sequence is an arginine. However, our sequence data (see FIG. 1) and Genebank accession numbers BC015850, NM_00948, X54393, V00566, M29386, D00411 and U75583 indicate that amino acid number 128 in hPRL is a glutamic acid. |
Claims (3)
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US20110129873A1 (en) * | 2008-04-30 | 2011-06-02 | Monsanto Technology Llc | Recombinant DNA Vectors for Expression of Human Prolactin Antagonists |
WO2013034116A1 (en) * | 2011-09-09 | 2013-03-14 | 清华大学 | Vascular endothelial myostatin mutant that mutates at atp binding sites |
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AU2003300904A1 (en) * | 2002-12-13 | 2004-07-09 | The Ohio State University | Antagonists for human prolactin |
AU2011213758B2 (en) * | 2004-02-19 | 2012-11-15 | Yale University | Identification of cancer protein biomarkers using proteomic techniques |
US7666583B2 (en) * | 2004-02-19 | 2010-02-23 | Yale University | Identification of cancer protein biomarkers using proteomic techniques |
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US20110129873A1 (en) * | 2008-04-30 | 2011-06-02 | Monsanto Technology Llc | Recombinant DNA Vectors for Expression of Human Prolactin Antagonists |
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AU2012306826B2 (en) * | 2011-09-09 | 2018-01-04 | Beijing Protgen Ltd. | Endostatin mutants with mutations at ATP binding sites |
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